IgG, Fc (Biotin)

Size

£314.00

SKU:: I1904-49B
Purity:: Purified by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Rat IgG F(c) fragment
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Biotin conjugated.
Extra Details:: Applications: Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.