IgG, H&L (Biotin)

Size

£646.00

SKU:: I1904-47S
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rat IgG whole molecule.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitution: Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Extra Details:: Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:20,000 to 1:100,000. 1ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Goat IgG molecule Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.