IgG, H&L, X-Adsorbed (AP)

Size

£623.00

SKU:: I1904-47P
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rat IgG whole molecule
Format:: Supplied as a liquid in Tris chloride, pH 8.0, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP) (Calf Intestine MW:140kD).
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:4000-1:20,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.