IgG, H&L (FITC)

Size

£429.00

SKU:: I1904-45U
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Human IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 2ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Recommended Dilutions: FLISA: 1:10,000-1:50,000 Immunofluorescence: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of Sheep IgG Absorption Wavelength: 495nm Emission Wavelength: 528nm Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.