IgG, F(ab’)2 X-Adsorbed (Biotin)

Size

£623.00

SKU:: I1904-45F
Purity:: Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human IgG F(ab’)2 fragment
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Biotin conjugated.
Extra Details:: Applications: Suitable foruse in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy. Other applications not tested. Recommended Dilutions: Standard Capture ELISA: 1:12,000 to 1:55,000, 1ug of Human IgG in a using Peroxidase Conjugated Streptavidin. Optimal dilution determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.