IgG (HRP)

Size

£699.00

SKU:: I1904-44S
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human IgG gamma chain.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Extra Details:: Applications: Suitable for use in Immunoblot (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:60,000-1:300,000. Use ABTS as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.