IgG, H&L (FITC)

Size

£557.00

SKU:: I1904-44L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (immunogloblin and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in FLISA, Fluorescent Western Blot, Immunofluorescence microscopy, Flow Cytometry and multiplex analysis. Other applications have not been tested. Recommended Dilution: FLISA: 1:10,000-1:50,000 Immunofluorescence Microscopy: 1:1000-1:5000 Flow Cytometry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Excitation: 495nm Emission: 528nm F/P Ratio: 3.6 Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.