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I1904-43

IgG, X-Adsorbed (Biotin)

Size

£479.00

SKU:
I1904-43
Purity:
Purified by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:
4°C (-20°C Glycerol)
Supplier:
United States Biological
Host:
Goat
Immunogen:
Rabbit IgG, whole molecule
Format:
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin
Extra Details:
Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:50,000-1:500,000. Assayed against 1ug of rabbit IgG in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 min. at RT. Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per goat IgG molecule Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice