IgG H&L (FITC)

Size

£553.00

SKU:: I1904-41Y
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rabbit IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW: 390D). Reconstitute with 1ml sterile dH2O. Absorption Wavelength: 495nm Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 3.4 moles FITC per mole of Goat IgG Fab.
Extra Details:: Applications: Suitable for use in FLISA, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: FLISA:1:10,000 - 1:50,000 Flow Cytometry: 1:500 - 1:2,500 IF Microscopy: 1:1,000 -1:5,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.