IgG, F(ab')2, X-Adsorbed (AP)

Size

£721.00

SKU:: I1904-41K
Purity:: Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rabbit IgG F(ab’)2 fragment
Format:: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Extra Details:: Applications: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Optimal dilutions to be determined by the researcher. Recommended Dilutions: This product has been assayed against 1ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:600 to 1:2,900 of the reconstitution concentration is suggested for this product. Optimal dilutions to be determined by the researcher. Storage Conditions: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice