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I1904-41C

IgG, F(ab')2 (Biotin)

Size

£435.00

SKU:
I1904-41C
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Goat
Immunogen:
Rabbit IgG F(ab’)2 fragment
Format:
Biotin conjugated. Lyophilized in 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4.Stabilizer: 10mg/ml BSA (IgG and Protease free). Preservative: 0.01% (w/v) Thimerosal.
Extra Details:
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule Application(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilution(s): This product has been assayed against 1ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:16,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Shipping Conditions:
Blue Ice