IgG, Fc, X-Adsorbed (HRP)

Size

£474.00

SKU:: I1904-40P
Purity:: Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rabbit IgG F(c) fragment
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Other applications not tested. Recommended Dilution: ELISA: 1:20,000 to 1:80,000 Standard ELISA. Western Blot:1:2000 to 1:10,000 Immunohistochemistry: 1:500 to 1:2500. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.