IgG, Fc (HRP)

Size

£524.00

SKU:: I1904-40J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Goat
Immunogen:: Rabbit IgG F(c) fragment.
Format:: Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:2000-1:7000 (Coat with 1ug rabbit IgG in standard sandwich ELISA). Optimal dilutions to be determined by the researcher. Label: Horseradish Peroxidase (HRP) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.