IgG, Rabbit (FITC) (Not For Export EU)

Size

£461.00

SKU:: I1904-38Y
Purity:: Purified from normal serum by delipidation, salt fractionation and ion exchange chromatography. IEP Assay shows a single precipitin arc against anti-fluorescein, anti-rabbit IgG and anti-rabbit serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) sodium azide. Reconstitute with 1ml dH2O or 40-50% glycerol. Labeled with Fluorescein isothiocyanate (FITC) (MW 390D). Absorption Wavelength:495nm. Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Rabbit IgG
Extra Details:: Applications: Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.