IgG, H&L (Biotin)

Size

£652.00

SKU:: I1904-34N
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Goat
Immunogen:: Human IgG whole molecule.
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Extra Details:: Applications: Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000-1:80,000; 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.