IgG, H&L (AP)

Size

£648.00

SKU:: I1904-34D
Purity:: Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Human IgG whole molecule
Format:: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Extra Details:: Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) Application(s): suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution(s): This product has been assayed against 1ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate NPP-10) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:9,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Shipping Conditions:: Blue Ice