IgG, H&L, X-adsorbed (Biotin)

Size

£576.00

SKU:: I1904-29V
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Goat IgG whole molecule
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Reconstitution: 0.5ml sterile dH2O.
Extra Details:: Applications: Suitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry. Other applications not tested. Recommended Dilution: Immunoblot: 1:10,000 ELISA: 1:40,000-1:200,000; 1.0ug of Goat IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT. mmunohistochemistry (tissue sections): 1:500-1:5000 Optimal dilutions to be determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.