IgG, H&L (HRP)

Size

£721.00

SKU:: I1904-29Q
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Donkey
Immunogen:: Goat IgG whole molecule
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Label: Horseradish Peroxidase (HRP) Reconstitution: 1.0ml sterile dH2O.
Extra Details:: Applications: Suitable for use in Immunoblot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:3000-1:13,000; 1.0ug of Goat IgG was used in a standard capture ELISA with ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.