I1904-28U
IgG, Fc (Biotin)
Size
£434.00
- SKU:
- I1904-28U
- Purity:
- Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Goat IgG F(c) fragment
- Format:
- Supplied as a liquid in 0.02 M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) thimerosal, 25% glycerol.
- Extra Details:
- Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG moleculeApplication(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilution(s): ELISA: 1:20,000 to 1:100,000. Assayed against 1ug of goat IgG in a standard capture ELISA using peroxidase conjugated streptavidin. A working dilution of of the reconstitution concentration is suggested for this product. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice