I1904-19A
IgG, F(ab’)2 (FITC or DTAF)
Size
£338.00
- SKU:
- I1904-19A
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Mouse IgG F(ab’)2 fragment.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC.
- Extra Details:
- DTAF has absorption and emission properties nearly identical to fluorescein isothiocyanate (FITC) and DTAF- and FITC-labeled antibodies are similar in terms of preparation and specificity of immunofluorescence. However, DTAF is superior to FITC with regard to cost, purity and stability. Also, DTAF-labeled rabbit IgG conjugates can be quickly and efficiently fractionated by simple ammonium sulfate precipitation to yield preparations free of both over- and under-conjugated material. In addition, over-conjugated protein can be readily removed from DTAF:IgG conjugates by appropriate adjustment of pH and temperature. Applications: Suitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot. Other applications not tested. Recommended Dilutions: FLISA: 1:10,000-1:50,000 Immunofluorescence: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Label (FITC): Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 3 moles FITC per mole of IgG Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice