IgG, H&L, X-adsorbed (HRP)

Size

£652.00

SKU:: I1904-09V
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Mouse IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with HRP (MW: 40kD). Reconstitute with 500ul sterile ddH2O.
Extra Details:: Applications: Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:20,000-1:100,000 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.