IgG, H&L, X-adsorbed (Biotin)

Size

£750.00

SKU:: I1904-09R
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Donkey
Immunogen:: Mouse IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 0.5ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
Extra Details:: Applications: Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested Recommended Dilution: ELISA: 1:25,000 to 1:100,000, using peroxidase conjugated streptavidin and ABTS for 30 minutes at RT. Optimal dilution determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules per donkey IgG molecule. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.