IgG, H&L (FITC)

Size

£544.00

SKU:: I1904-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Mouse IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in Immunofluorescence, Fluorescent Western Blot and FLISA (Fluorescent). Other applications have not been tested. Recommended Dilutions: Immunofluorescence: 1:1000-1:5000 FLISA (Fluorescent): 1:10,000-1:50,000 Optimal dilution determined by the researcher. Excitation Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 3.5 moles of FITC/mole of chicken IgG Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.