IgG, Human, Non-Immune Control (Not For Export EU)

Size

£607.00

SKU:: I1903-90P4
Purity:: Highly Purified (~95%)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 1ml sterile PBS.
Extra Details:: A healthy non-immunized population of humans was used to collect serum. IgG was prepared by ammonium sulfate, and column chromatography (Sephadex, and DEAE-ion exchange columns). The resulting preparation was judged to be >95% pure by IEP and SDS-Page. Source: Human normal serum. Applications: Suitable for use in ELISA (coating), or as a non-immune control for ELISA, Dot Blot, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 10ug/ml Dot Blot: 10ug/ml Western Blot: 10ug/ml Immunohistochemistry: 10ug/ml Optimal dilutions to be determined by the researcher Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.