IgG, Fc (HRP)

Size

£576.00

SKU:: I1903-38Y
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human IgG F(c) fragment
Format:: Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in Immunoblot (Western or dot blot), ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:8000, Use ABTS as a substrate for 30 min at RT to assay against 1ug of human IgG. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.