IgG, H&L (Biotin)

Size

£648.00

SKU:: I1903-32E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Guinea Pig IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerisol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in Western Blot, ELISA, Immunomicroscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:20,000; Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.