IgG, F(ab’)2 (FITC)

Size

£492.00

SKU:: I1903-31F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Guinea Pig IgG F(ab’)2 fragment
Format:: Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute in 1ml sterile ddH2O. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Applications: Suitable for use in FLISA, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: FLISA: 1:10,000-1:50,000 Immunofluorescence: 1:1,000-1:5000 Flow Cytometry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 2.8 moles FITC per mole of Goat IgG Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap