IgG, H&L (FITC)

Size

£442.00

SKU:: I1903-31
Purity:: Purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine and rabbit serum to eliminate crossreactivity.
Storage Conditions:: 4°C
Supplier:: United States Biological
Host:: Donkey
Immunogen:: Sheep IgG
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Applications: For use as a secondary developing reagent in immunofluorescence assays including immunohistochemistry, immunocytochemistry, flow cytometry, and other fluorescent immunoassays with unlabeled primary antibodies of sheep origin. Reagent Preparation: Centrifuge the antibody solution in a microfuge before use. For most applications, a dilution of 1:200 is recommended. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.