IgG, H&L, X-adsorbed (HRP)

Size

£691.00

SKU:: I1903-30U
Purity:: Purified by immunoaffinity chromatography followed by solid phase adsorption.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Rabbit IgG, whole molecule
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH20
Extra Details:: Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 1:8000 -1:40,000. Assayed against 1ug of rabbit IgG in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT. Western Blot: 1:1000 -10,000 Immunohistochemistry: 1:500 -1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.