IgG, H&L, X-adsorbed (Biotin)

Size

£646.00

SKU:: I1903-30S
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rat
Immunogen:: Rabbit IgG whole molecule
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per rat IgG molecule.
Extra Details:: Applications: Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested Recommended Dilutions: ELISA: 1:12,000 to 1:52,000. Assayed against 1ug raggit IgG in a standard ELISA with ABTS (A0030) as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.