IgG, H&L, X-Adsorbed (Biotin)

Size

£646.00

SKU:: I1903-30C
Purity:: Purified by immunoaffinity chromatography using goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, goat IgG, anti-biotin and goat serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Goat IgG whole molecule (H&L)
Format:: Supplied as a lyophilized powder or liquid in PBS, pH 7.2, BSA, 0.01% sodium azide. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Western Blot, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilution: ELISA: 1:28,000-1:116,000 using ABTS as substrate detects 1ug of Goat IgG in standard sandwich ELISA.. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.