IgG, H&L (HRP)

Size

£437.00

SKU:: I1903-21U
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Horse IgG whole molecule Label: Horseradish Peroxidase (HRP)
Format:: Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA,(IgG and protease free), 0.01% Gentamicin Sulfate, 40% glycerol.
Extra Details:: Applications: Suitable for immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (standard capture): 1:3,000-1:20,000, 1.0 ug of Horse IgG using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.