IgG, Fc (HRP)

Size

£524.00

SKU:: I1903-19J
Purity:: Purified byimmunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: horseIgG F(c) fragment
Format:: Supplied as a liquid in PBS pH 7.2, 10 mg/ml BSA, (IgG and Protease free) 0.01% Gentamicin Sulfate, 40% glycerol. Label: Horseradish Peroxidase (HRP)
Extra Details:: Applications: Suitable for use in immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (standard capture): 1:7,500-1:36,000, 1.0 ug of Horse IgG using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.