IgG, H&L (Biotin)

Size

£448.00

SKU:: I1903-17E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Dog IgG whole molecule
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Extra Details:: Applications: Suitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:60,000; 1.0ug of Canine IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.