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I1903-14S

IgG, H&L (AP)

Size

£579.00

SKU:
I1903-14S
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Sheep
Immunogen:
Rat IgG whole molecule
Format:
Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD)
Extra Details:
Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 to 1:5000. 1.0 ug of rat IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice