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I1903-13K

IgG, H&L, X-adsorbed (Biotin)

Size

£641.00

SKU:
I1903-13K
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Donkey
Immunogen:
Rat IgG whole molecule.
Format:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin.
Extra Details:
Applications: Suitable for ELISA, IWestern Blot and Immunohistochemistry. Other applications not tested Recommended Dilutions: ELISA: 1:20,000-1:140,000. Assayed against 1ug of rat IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT. Immunohistochemistry: 1:1000-1:5000 Western Blot: 1:2000-1:10,000 Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice