I1903-03E
IgG, Fc (Biotin)
Size
£544.00
- SKU:
- I1903-03E
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Feline IgG F(c) fragment.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease free, 0.01% sodium azide,. Labeled with Biotin. Reconstitute with 1mL sterile ddH2O.
- Extra Details:
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Cat IgG F(c) antibody is ideal for investigators in Immunology, Cancer, and Microbiology research. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry.. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000 to 1:220,000. Assayed against 1ug of feline IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Western Blot: 1:2000-1:10,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice