IgE (epsilon Chain) (FITC)

Size

£448.00

SKU:: I1900-42E5
Purity:: Purified by affinity chromatography. ≥95% by SDS-PAGE.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified human IgE, epsilon Chain
Format:: Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
Extra Details:: Applications: Suitable for use in FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher. Absorption: 494nm Emission: 518nm FITC/Protein Ratio: 7 Moles FITC per Mole Antibody Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.