IgG (HRP)

Size

£479.00

SKU:: I1900-30C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Rat IgG whole molecule.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:500-1:2500 ELISA: 1;60.000-1:100,000. Assayed against 1ug of rat IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Western Blot: 1:1000-1:10,000 Optimal working dilutions to be determined by researcher. Label Molecular Weight: 40,000 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C . Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.