I0706-94B
Iduronidase, a-L- (alpha-L-Iduronidase, IDUA)
Size
£934.00
- SKU:
- I0706-94B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ala26-Pro653 from human alpha-L-Iduronidase/IDUA, expressed in NSO cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Alpha-L-Iduronidase (IDUA) is a ubiquitously expressed lysosomal glycosyl hydrolase. It hydrolyzes the unsulfated terminal alpha-L-Iduronic acid residues in glycosaminoglycans including dermatan sulfate and heparin sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis. Applications: Suitable for use in Direct ELISA, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Human kidney tissue and IMR human lung fibroblast cell line Immunocytochemistry: 5-15ug/ml; immersion fixed HepG2 human hepatocellular carcinoma cell line Immunoprecipitation: 25ug/ml; conditioned cell culture medium spiked with recombinant human IDUA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice