I0706-94

Iduronidase, a-L-, Recombinant, Human, aa26-653, His-Tag (a-L-Iduronidase, IDUA)

Size

£1,017.00

SKU:: I0706-94
Purity:: ≥95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM sodium acetate, 400mM sodium chloride, pH 5.0, 20% glycerol.
Extra Details:: Alpha-L-Iduronidase encoded by the IDUA gene is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGs). It hydrolyzes the non-reducing terminal alpha-L-iduronic acid residues in GAGs including dermatan sulfate and heparan sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I) (1). MPS I causes progressive cellular, tissue and organ damage, and several clinical studies using enzyme replacement therapy have shown promising benefits (2). The purified rhIDUA has an N-terminal sequence of T26EAPHLVQVD. The 638 amino acid residue rhIDUA predicts a molecular mass of 71kD, and migrates as an ~83kD protein by SDS-PAGE under reducing conditions. Recombinant protein corresponding to Ala26-Pro653 (Ala26Thr) with a C-terminal 10-His tag expressed in NSO cells (AAA81589). Molecular Mass: The purified rhIDUA has an N-terminal sequence of T26EAPHLVQVD. The 638 amino acid residue rhIDUA predicts a molecular mass of 71kD, and migrates as an approximately 83kD protein by SDS-PAGE under reducing conditions. Activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha-L-iduronide. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice