I0650-01H

ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein Gl50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7 H2 ICOS Ligand)

Size

£1,004.00

SKU:: I0650-01H
Purity:: Purified by Protein G affinity chromatography from cell culture supernatant. Endotoxin: <0.1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Asp19-Ser258 from human ICOS Ligand, expressed in NS0 cells.
Clone:: 8H103
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Extra Details:: ICOS Ligand, also known as B7-related protein 1 (B7RP-1), is a costimulatory molecule that serves as a ligand for ICOS (Inducible Co-Stimulator). Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Neutralization: 1-4ug/ml yields one-half maximal inhibition (ND50) of the cell surface ICOS Ligand in the presence of 20ng/ml of anti-human CD3 and 3ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA activated human T cells as an assay. Western Blot: 1ug/ml with recombinant human B7-H2 Fc Chimera Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.