H9810-01J
Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha, Hypoxia Inducible Factor 2 alpha Subunit, HIF-2a, bHLHe73, ECYT4, Endothelial PAS Domain Containing Protein 1, Endothelial Pas Domain Protein 1, EPAS1, EPAS-1, HIF-2 alpha, HIF2a, HIF1 alpha-like Factor, HLF, Member of PAS Protein 2, Member of Pas Superfamily 2, MOP2, PASD2)
Size
£812.00
- SKU:
- H9810-01J
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser543-Thr870 from human HIF-2-alpha, expressed in E.coli.
- Clone:
- 11C21
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- HIF-2-alpha/EPAS1 (hypoxia-inducible transcription factor 2-alpha/enodothelial PAS domain 1) is stabilized in hypoxic tissue and, similarly to HIF-1-alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1-alpha and HIF-2-alpha. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using 786-O human renal cell adenocarcinoma cell line (HIF-2 alpha positive), Caki-2 human clear cell carcinoma epithelial cell line (HIF-2alpha negative), and HeLa human cervical epithelial carcinoma cell line (HIF-2-alpha negative) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice