H9807-04
Hypoxia Inducible Factor 1a (HIF-1a)
Size
£665.00
- SKU:
- H9807-04
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Fusion protein corresponding to the internal region, aa432-528, of human HIF-1a
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- Hypoxia Inducible Factor 1 (HIF-1) is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. Applications: Suitable for use in Western Blot, Gel Shift Assays, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot:1:500-1:1000 Immunoprecipitation: 1:1000 Chromatin Immunoprecipitation: 25ul for 15 million cells. Nuclear extracts are recommended. Immunohistochemistry (Frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:100-1:300 Requires HIER using sodium citrate buffer pH6.0 Mixed results with mouse. Gel Shift Assay: 1:1-1:100 Immunofluorescence (IC): 1:100-1:500 Optimal dilutions to be determined by the researcher. Positive Controls: RCC4 lysates, COS-7 hypoxic induced control lysate, PC12 nuclear extracts. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice