H9804-01B

Hypoxia Inducible Factor 1 alpha, Recombinant, Human, aa530-826 (HIF-1a)

Size

£364.00

SKU:: H9804-01B
Molecular Weight:: 32.8
Purity:: ≥ 90% (SDS-PAGE). Purified by conventional chromatography techniques.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1alpha, HIF-2alpha or HIF-3alpha). The activation of HIF-1alpha is closely associated with a variety of tumors and oncogenic pathways. HIF-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminal regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. HIF-1alpha (aa530-826) contains two TAD and inhibitory domain. Recombinant protein corresponding to aa530-826 from human Hypoxia Inducible Factor 1 alpha, expressed in E. coli. Swiss/Uniprot: Q16665 Molecular Weight: ~32.8kD (298aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) Amino Acid Sequence: MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice