H9117-40E

HSD11B1 (Corticosteroid 11-beta-dehydrogenase Isozyme 1, 11-beta-hydroxysteroid Dehydrogenase 1, 11-DH, 11-beta-HSD1, HSD11, HSD11L)

Size

£642.00

SKU:: H9117-40E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to CTSYNMDRFINK, from human HSD11B1, at C-terminal (NP_005516.1, NP_861420.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:8000 Western Blot: 0.3-1ug/ml, observed in human liver lysates on ~35kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.