H9117-40A
Hydroxysteroid Dehydrogenase, 11-beta, Type I (HSD)
Size
£617.00
- SKU:
- H9117-40A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the internal region of human 11β-HSD1.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Hydroxysteroid Dehydrogenase, 11-beta, Type-1 catalyzes the conversion of inactive cortisone to active cortisol in adipose tissue. Its expression more closely parallels that of the glucocorticoid receptor than that of the mineralocorticoid receptor, to regulate the exposure of cortisol to glucocorticoid receptors. mRNA and protein of this enzyme have been detected in a wide range of tissues including liver, kidney, testis, lung, heart and colon. The enzyme’s expression is developmentally regulated, suggesting its importance in regulation of glucocoricoid action during embryonic development. Over-expression of 11beta-HSD1 results in visceral obesity and metabolic syndrome including insulin-resistant diabetes, hyperlipidemia and hyperphagia. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:200 Immunohistochemistry (Formalin-fixed paraffin-emedded sections): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice