H9042-07

Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1, HSD3B, HSDB3)

Size

£642.00

SKU:: H9042-07
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-DRHKETLKSKTQ, from the C-terminus of human HSD3B1 (NP_000853.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:128,000 Western Blot: 0.01-0.1ug/ml detects ~40kD band observed in Human Placenta lysates (calculated MW of 42.3kD according to NP_000853.1). In transfected HEK293 transiently expressing Human HSD3B1 a band of approx. 45kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.