Huntingtin Protein, phosphorylated (Ser421) (Huntington Disease)

Size

£914.00

SKU:: H7965-03
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa416-424, R-S-R-S-G-pS-I-V-E of the human huntingtin gene protein (mutations cause Huntington's disease poly-GLU expansions causing plaques)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Extra Details:: Huntingtin disease, a neurodegenerate disease, is caused by the expansion of a polymorphic glutamine tract in huntingtin. The Huntingtin Interacting Protein 1 (HIP-1) is a reportedly proapoptotic, cargo-specific adaptor protein that may be involved in the pathogenesis of Huntingtin disease. As well as playing a role in Huntingtin disease, it is likely to be involved in the recruitment of clathrin coats to lipid membranes and it may also factor in tumorigenesis by allowing the survival of precancerous and cancerous cells. Since HIP-1 expression is significantly associated with prostate and colon cancer metastasis, HIP-1 can serve as a putative prognostic factor for prostate and colon cancers. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:40,000 Western Blot: 1:500- 1:3000. Detects bands at ~350 and ~200kD. Immunohistochemistry: 1:50- 1:100 Optimal dilutions to be determined by the researcher. Recommended Control Peptides: H7965-03A: Huntington pS421, phosphorylated, CONTROL PEPTIDE (416-424) H7965-03B: Huntingtin Protein, Non-phosphorylated, CONTROL PEPTIDE (416-424) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice