HuR (Hu antigen R, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1, ELAV1, ELAV-like protein 1, Hua, Hu-antigen R, HUR, MelG) (HRP)

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£1,042.00

SKU:: H7953-34B-HRP
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminus of human HuR.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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